Determining The Linear Range For Quantitative Western Blot Detection

Accurate quantification in Western blotting hinges on detecting both target proteins and internal loading controls within a shared linear range. This manual offers a step-by-step guide to experimentally determine that range using either total protein stains or housekeeping proteins. It explains how to identify saturation points, avoid signal distortion, and ensure proportional signal responses across sample dilutions. Whether using Revert™ 700 Total Protein Stain or a housekeeping protein, researchers can learn how to select optimal sample loading amounts to maintain linearity and improve normalization accuracy. For those working with post-translational modifications or highly variable protein expression, gain insight into alternative strategies and validation steps.

Access the full manual to improve the reliability of your quantitative Western blot results.