Detection Of Adapter Dimers In NGS Libraries With The Agilent Fragment Analyzer And TapeStation Systems

Accurate detection of adapter dimers is essential for achieving high‑quality NGS results, especially as sequencing platforms become more sensitive and less tolerant of small-fragment contaminants. Explore how electrophoresis-based QC enables researchers to identify even trace levels of adapter dimers that can otherwise distort library quantification, reduce sequencing diversity, or consume a disproportionate share of reads. Through side‑by‑side evaluations of different electrophoresis kits and assays, see how varying sensitivities and resolution levels impact the ability to distinguish true library fragments from unwanted dimer products. The findings offer practical guidance for selecting the right QC approach for DNA and RNA‑Seq workflows, ensuring cleaner libraries and more reliable sequencing output.

Dive into the full application note to compare kit performance and determine which method best fits your workflow.