Designing An Advanced Protein A Chromatography Resin
Watch It On Demand
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The relationships and considerations of bead structure, pore structure, surface chemistries and ligand design on affinity resin performance targets.
Industry needs, patent expirations of ligand design and market dynamics have accelerated the development and availability of many protein affinity resins. Technology providers must consider a range of design parameters such as resin backbone, bead size, pore size, surface chemistry modification, ligand construct and how they relate to performance criteria such as DBC, life time, caustic stability, and column packing among others. JSR’s approach to designing Amsphere™ A3 affinity resin will be discussed. The trade-offs between resin design and resin performance will be illustrated through a case study.
Key Learning Objectives:
- Understand the rationale behind the design of a Protein A resin
- Understand the relationships of bead design, ligand design and surface modifications on resin performance
- Review a case study comparing 4 mAbs and 4 commercial resin types of commercial resins
- Review mAb process productivity analysis of Ampshere A3
Presenters:
Marty Siwak , JSR Life Sciences, Director, Separations Sciences Group
Zhuo Liu, KBI Biopharma, Process Development Scientist II