Cyto-Chex® Blood Collection Tube Stabilizes Samples Stored At Elevated Temperatures For Flow Cytometry Analysis
Poster: Cyto-Chex® Blood Collection Tube Stabilizes Samples Stored At Elevated Temperatures For Flow Cytometry Analysis
Dominic E. Warrino, Jodi R. Alt, James A. Grunkemeyer, and Wayne L. Ryan Streck, Omaha, NE 68128
Abstract
We have previously demonstrated that Streck Cyto-Chex® Blood Collection Tube
(BCT) preserves whole blood specimens for HIV testing by flow cytometric
analysis for seven days at room temperature. Increased stability reduces the
need for weekend staffing and shipping delays are not likely to require patient
redraws. However, in resource poor settings sample storage, transportation time,
and temperature are not reliable. The effect of elevated temperatures on
stabilized blood specimens collected in Cyto-Chex BCT has not been tested.
Here, we report that Cyto-Chex BCT is capable of maintaining the integrity of CD
markers critical for HIV assessment at temperatures up to 37°C for three days.
Whole blood samples were drawn from 25 healthy donors into K2EDTA tubes
and Cyto-Chex BCT. Flow cytometric analysis was performed on samples
collected and stored at 30°C or 37°C for 6 hours, 3 days, or 7 days. Cell counts
for CD3, CD4, and CD8 were determined for each sample at each time point and
compared to 6 hour K2EDTA samples. While K2EDTA samples fail at elevated
temperatures, Cyto-Chex BCT stabilizes all three markers for seven days at
30°C. Additionally, CD3 and CD4 are stable at 37°C for 72 hours. Cyto-Chex
BCT can increase the reliability of CD4 testing in resource poor areas. Monitoring
CD4 counts in HIV patients is an extremely important tool enabling physicians to
modulate treatment accordingly. Streck Cyto-Chex BCT stabilizes whole blood
samples at elevated temperatures for extended periods of time, thereby
improving the reliability of HIV monitoring.
Poster: Cyto-Chex® Blood Collection Tube Stabilizes Samples Stored At Elevated Temperatures For Flow Cytometry Analysis
SOURCE: Streck, Inc.