Cultivate CHO Cell Culture In The Multitron With Shaker Flasks

The Multitron incubation shaker from INFORS HT is particularly well-suited for the production of seed cultures for inoculation, as well as for the production of the Secreted Alkaline Phosphatase (SEAP) protein. To produce seed cultures for inoculation and for the production of the SEAP protein, the CHO XM-111 clone was transfected with an expression vector that codes for the gene of the recombinant protein SEAP. The use of expression vectors allows for the selectable expression of two genes using a single promoter. This allows for the production of SEAP in a two-phase process: a non-productive growth phase followed by a proliferation-inhibited production phase based on the media exchange without tetracycline.

In simpler terms, the use of incubation shakers is making it easier and more efficient to grow animal cells in the lab. This is important for biotechnology research and production.