Confident O-Glycosylation Site Identification For ENBREL (etanercept) Using The ECD Functionality Of SELECT SERIES Cyclic IMS System

By Samantha Ippoliti, Dale Cooper-Shepherd, Ying Qing Yu, James I. Langridge

Characterization of glycosylation on the glycopeptide level can be challenging with traditional peptide mapping methods that employ reversed-phase liquid chromatography coupled with tandem mass spectrometry. These methods typically employ collision induced dissociation as the fragmentation technique, which has limitations for site specific analysis of labile modifications. The use of Electron Capture Dissociation (ECD) enables glycan moieties to be retained on the peptide backbone and allows the site to be confidently assigned using the resulting fragment ions.

Explore the utility of ECD on the SELECT SERIES Cyclic IMS instrument for unambiguous assignment of glycosylation site locations for 11 Oglycopeptide species of ENBREL (etanercept).