A Comprehensive Workflow To Characterize Deglycosylated NISTmAb Using icIEF-UV/MS

By Rashmi Madda, Jingwen Ding, Mariam ElNaggar, Scott Mack, Maggie Ostrowski and Zoe Zhang SCIEX, USA

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Examine a comprehensive analysis of deglycosylated NISTmAb using a novel integrated icIEF-UV/MS workflow. Explore the streamlined platform offering confident characterization and reducing the time waiting to obtain results to approximately 1 hour for routine samples. This deglycosylation experiment in this technical note demonstrates the capability of the Intabio ZT system to confidently identify glycation events using icIEF-UV/MS and how a unified icIEF-UV/MS workflow offers high sensitivity and selectivity to detect low-abundant proteoforms that might impact product quality.