Comprehensive Phosphoramidite Chemistry Capabilities For The Synthesis Of Oligonucleotides

Natural DNA is composed of nucleotides arranged in repeating units that form a chemical chain, with each nucleotide linked through enzymatic activity. The creation of synthetic DNA relies on replicating this natural process while enabling precise assembly of custom oligonucleotide sequences. The industry-standard method for this is Phosphoramidite chemistry, a validated technology that has enabled efficient oligonucleotide synthesis for nearly 35 years.

In the past five years alone, 11 oligonucleotide-based therapies have gained approval, with over 100 currently used in diagnostics and more than 100 others undergoing clinical trials. Thanks to the high efficiency of phosphoramidite chemistry, large-scale synthesis of oligonucleotide sequences—up to 200 base pairs in length—is now routinely achievable.

Aragen Life Sciences is a leading provider of custom phosphoramidite synthesis, offering tailored solutions to meet diverse research, diagnostic, and therapeutic needs. Our chemists specialize in synthesizing a broad range of phosphoramidites featuring various protecting groups and chemical modifications. We support the incorporation of custom functionalities — such as fluorophores, labels, or other chemical moieties — into the phosphoramidite backbone.

Aragen also offers scalable synthesis capabilities, ranging from milligram to multi-gram quantities, depending on client requirements. Comprehensive quality control is integral to our process, including advanced analytical techniques such as NMR spectroscopy, HPLC, and mass spectrometry to ensure the structural integrity and purity of every product.