Column Selection For RPLC-UV Impurity Analysis Of Fatty Acid Modified GLP-1 Receptor Agonists
By Caitlin M. Hanna, Stephan M. Koza, and Steve Shiner, Waters Corporation
In this application note, evaluate and compare four different column chemistries to determine their suitability for reversed-phase liquid chromatography with ultraviolet detection (RPLC-UV) in the impurity analysis of two fatty acid-modified GLP-1 receptor agonists: liraglutide and semaglutide. The chromatographic conditions and column selections presented serve as a practical guide for method development, which offers analyte-specific insights that can be tailored to the unique structural and chemical characteristics of these peptide-based therapeutics. This work provides a foundational framework for optimizing RPLC-UV methodologies to ensure robust and reliable impurity profiling for this important class of biopharmaceuticals.
Access the full application note to learn more.
Get unlimited access to:
Enter your credentials below to log in. Not yet a member of Bioprocess Online? Subscribe today.