Application Note

Column Packing Protocol For Amsphere A3

Source: JSR Life Sciences
A Lifetime Study For Protein A Resin With CIP Cycle Testing

This packing protocol is for small columns (1-20 mL, ID=0.5-1 cm). Recommended packing method is by flow.

The slurry of Amsphere A3 is provided in a storage buffer solution containing 16% ethanol in 20 mM sodium phosphate, pH 7.5. Decant the storage solution once the resin has settled, and add 0.1 M sodium chloride to obtain a slurry concentration of 50% (v/v) (buffers other than 0.1 M sodium chloride can be used but could influence the packing quality).