Collagen Antibodies
Code: 600-401-104-0.1
Lot # 7066
Size: 100 g
Physical State: Liquid (sterile filtered)
Antibody Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
Buffer: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; pH 8.0
Stabilizer: None
Preservative: None
Application(s): Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Recommended Dilution(s): This product was assayed by immunoblot and found to be reactive against Collagen II at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 ug of Collagen II in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher.
Storage Conditions: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
Purity and Specificity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen: Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Rockland Immunochemicals Inc., P.O. Box 316, Gilbertsville, PA 19525. Tel: 610-369-1008; Fax: 610-367-7825.