Cloning Kit

The Perfectly Blunt Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, which decreases the probability of generating mutations in the target sequence.

The protocol allows you to go from PCR product to plating transformants in less than one hour with minimal hands-on time. The finished PCR reaction product is converted to a blunt, phosphorylated form in a 15-minute reaction using premixed reagents. Following a 5-minute heat inactivation step, the treated insert is combined with the ready-to-use vector and ligated in an optimized 15-minute reaction. An 8-minute transformation procedure using NovaBlue Singles Competent Cells generates recombinant colonies that are easily visualized by blue/white screening.

Novagen, Inc., 601 Science Drive, Madison, WI 53711. Tel: 608-238-6110; Fax: 608-238-1388.