Cleaning Protocols To Reduce The Effects Of Pressure Rise Over The Lifetime Of A Gore Protein Capture Device

By Jared Clinger, B.S. and William C. Barrett, Ph.D., W.L. Gore & Associates, Inc.

The GORE Protein Capture Device improved potential processing capabilities for affinity Protein A chromatography, this shortening the time needed to purify a given monoclonal antibody. Increased speed and capacity allows for more purification life cycles in a given amount of time. Therefore, the lifetime of a device might be reached more rapidly that Protein A resins with less capacity and slower speeds to purification. Two cleaning protocols outlined in this application note potentially extend the lifetime of a GORE Protein Capture Device in the event that pressure elevates over the expected lifetime of the device.