Chromatography Training Series - Fundamentals Of Ion Exchange Chromatography

Ion exchange chromatography (IEX) is a powerful and widely used purification technique that separates biomolecules based on their charge characteristics. Commonly applied in the purification of proteins, antibodies, and biologics, IEX delivers high resolution, high recovery, and excellent selectivity across capture, intermediate purification, and polishing workflows.

This introductory training provides a comprehensive overview of ion exchange chromatography principles and their application in modern bioprocessing. Through expert-led instruction, learners will explore how protein charge, pH, buffer selection, and resin chemistry influence purification performance, while gaining practical insights into process development and optimization.

Whether you're new to downstream processing or looking to strengthen your chromatography knowledge, this course offers foundational concepts that can be applied across research, process development, and commercial biomanufacturing environments.

What You'll Learn

• The fundamental principles of ion exchange chromatography and charge-based biomolecule separation.
• The differences between anion exchange (AEX) and cation exchange (CEX) chromatography and when to use each approach.
• How protein charge and isoelectric point (pI) influence binding, selectivity, and purification strategy.
• Key chromatography concepts including dynamic binding capacity, residence time, resolution, selectivity, efficiency, HETP, and asymmetry.
• Best practices for selecting chromatographic resins, buffer systems, and operating conditions.
• The role of pH, conductivity, ionic strength, and salt gradients in protein binding and elution.
• How IEX is applied throughout bioprocessing workflows for capture, purification, and polishing of biologics.
• Strategies for optimizing yield, purity, contaminant removal, and process productivity.
• Practical considerations for column sizing, scale-up, cleaning, sanitization, and resin reuse.
• How modern chromatography technologies and advanced resin designs can improve resolution, efficiency, and sustainability.

Start the training today and build a stronger understanding of ion exchange chromatography and its critical role in the purification of proteins, monoclonal antibodies, and other biotherapeutic molecules. Access the full video course and additional chromatography learning resources through our training platform.