CHO Cell Culture Optimization: A Design Of Experiments Approach In The Ambr® 15

By Miroslav Matev, Shiori Akiyama, Toru Tsuji, and Satoshi Igarashi, Sartorius

Explore the impact of systematic process development on CHO cell culture optimization for high-yield monoclonal antibody production. Central to the study is the use of an automated microbioreactor system paired with Design of Experiments (DOE) software to streamline the optimization of pH, seeding density, and feeding strategies. Through a series of methodical trials, researchers identified the optimal media and feed combination for boosting titer and viability. The optimized process achieved a predicted IgG1 titer of 5.8 g/L in just 12 days, significantly outperforming shake flask cultures in both yield and consistency. By enabling rapid screening and precise parameter control, this platform proved to be a valuable tool for accelerating upstream development.

This application note reinforces the value of high-throughput, automated systems for reducing development timelines while enhancing reproducibility in biopharmaceutical manufacturing.