CHEF1™ Expression Technology

The CHEF1 expression system is widely used for clinical production cell lines and commercial manufacturing. It accelerates cGMP production by rapidly generating high levels of recombinant proteins using robust procedures and reliable materials. CHEF1 allows quick isolation of CHO cell lines expressing desired proteins without gene amplification, reducing development timelines. It uses adaptable CHO cells, chemically defined media, and specific plasmids. The system's vectors have selectable markers for multiple transfections and co-expression of post-translationally modified proteins. This eliminates methotrexate use and expedites early clinical trials. The CHEF1 expression platform technology incorporates suspension-adapted CHO DG44 cells, CD-CIM1 media, and vectors to produce stable expression pools in five weeks, yielding over 10 grams of protein. Cell line cloning is serum-free and progresses rapidly from plates to flasks. Early productivity screens predict expression potential, enabling swift cell line development. The CHEF1 expression platform ensures reliable manufacturing productivity during large-scale production. All development activities are well-documented for seamless transfer to cGMP production, facilitating quick entry into clinical trials. In summary, the CHEF1 expression system is an efficient and reliable system for creating cell lines and producing recombinant proteins.