Characterization And Relative Quantification Of mRNA 5'-Capping

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Review the development of an LC-MS method for the reliable identification and relative quantification of different mRNA capping structures. The method can distinguish between uncapped and multiple different capped 5’-ends of mRNA simultaneously, addressing the need for characterizing this CQA.

Key features of mRNA capping assessment with SCIEX technology

• Fast and reliable identification of the 5’-end structure of IVT mRNA species
• Straightforward and fully customizable quantification of mRNA capping efficiency through reconstructed masses in SCIEX OS software
• High confidence in identification of main and low-abundance species based on high-quality accurate mass data in negative polarity