Review the development of an LC-MS method for the reliable identification and relative quantification of different mRNA capping structures. The method can distinguish between uncapped and multiple different capped 5’-ends of mRNA simultaneously, addressing the need for characterizing this CQA.
Key features of mRNA capping assessment with SCIEX technology
- Fast and reliable identification of the 5’-end structure of IVT mRNA species
- Straightforward and fully customizable quantification of mRNA capping efficiency through reconstructed masses in SCIEX OS software
- High confidence in identification of main and low-abundance species based on high-quality accurate mass data in negative polarity