Challenges And Considerations For Purifying Multispecific Molecules

The structural complexity and variability of multispecific molecules presented purification challenges, from incorrect chain pairings to increased aggregation, fragmentation, and lower yield compared to standard mAbs. Developers shared how they overcame these challenges to achieve high purity and discussed key considerations for downstream developers when designing these processes. Bioprocess Online editor Tyler Menichiello explored these questions with Jian Ren, Ph.D., a principal scientist in downstream process development at AbbVie, and Mark Fitchmun, founder, president, and CEO of Somatek.