CESI 8000 High Performance Separation-ESI Module with OptiMS Technology

Extending the Reach of Mass Spectrometry

Mass spectrometry (MS) has become an indispensable technology for the analysis of compounds of biological interest. Whether you seek to characterize a therapeutic protein; identify the proteins that make up a specific proteome; characterize post-translational modifications; study a metabolomic fingerprint related to a particular condition; or quantify drugs and their metabolites in a minute or complex sample matrix, improvements in assay sensitivity and reductions in ion suppression serve to reveal new information. To achieve this we have developed a new front-end separation-and ionization technology called CESI, which combines the high efficiency and ultra-low flow characteristics of capillary electrophoresis with an integrated electrospray ionization source. Reduction of flow with electrospray ionization (ESI) into the nanoflow region has been reported to improve assay sensitivity (1) and reduce ion suppression (2) with ESI-MS. Of course, capillary electrophoresis is, by its very nature, an ultra-low flow separation technology.

• Increased Sensitivity – In order of magnitudes depending on analyte and MS
• High Efficiency Separation – Peak capacity >300
• Decreased Cycle Time – Short analysis and regeneration times
• Reduced Ion Suppression – Nanoflow leads to higher sensitivity
• Minute Sampling – From nano-litre to micro-litre sample introduction
• Broader Analyte Coverage – Ideal for highly charged and polar analytes

Capillary Electrophoresis (CE) is intrinsically a low flow technology that is known for its unmatched resolving power. The CESI 8000 High Performance Separation-ESI Module is designed for mass spectrometry applications that analyze charged and polar molecules. Thanks to its robust OptiMS technology, the advantages of Capillary Electrophoresis are now delivered to the mass spectrometer without dilution or disturbance. Stable ESI is generated at an ultra low flow in the low nL/min. range, resulting in ultra high sensitivity. These flow rates serve to reduce ion suppression effects.

UNSPSC: 41105307 Width: 25 in (63.5 cm) Height: 29.2 in (74.2 cm) Height with Door Open: 38.8 in (98.6 cm) Depth: 28.4 in (72.1 cm) Weight: 188 lbs (85.3 kg) includes photodiode array detection Electrical Requirements: Voltage 100 - 240 V 50/60 Hz Buffer Tray: 2 x 36 2 mL vials, 4 x 25 mL trays Current Range: 3 to 300 µA programmable at 0.1 µA Increments Pressure Range: -5 to 100 psi Sample Tray: 2 x 96-well plates, 2 x 48 2 mL vials, 2 x 48 0.5 mL vials, 2 x 48 PCR vials Voltage Range: 1 - 30 kV (1 - 10 kV for injections), Programmable in 0.1 kV increments, Reversible voltage programmed through user interface.

The CESI 8000 software simply requires the routine user to select one of the laboratory's pre-loaded methods, set the number of samples, load the reagents, and start running.

The CESI software will alert the mass spectrometry software to start collecting data at the appropriate time.

Direct control over the CESI conditions can be exerted at appropriate times.

1) Select Pre-Loaded Application

The CESI 8000 software simply requires the routine user to select one of the laboratory's pre-loaded methods, set the number of samples, load the reagents, and start running.

2) Set number of samples and load reagents

CESI 8000 software is graphical and very easy to work with. All vial positions may be annotated, allowing you to easily track the contents of any given vial.

3) Run

Either programmed control or direct control can be implemented, allowing the user maximum flexibility to optimize the separation.

CESI 8000 software features include:

• Automated sample handling from microvials or 96-well microplates
• Simple interfacing, with automatic triggering of MS data collection
• Easy switching between liquid chromatography (LC) and CESI-MS
• Enhanced Help menu and instructional videos
• On-screen prompts for monitoring system events
• Advanced reporting capability
• Increased workspace