Product/Service

cDNA Libraries

Source: OriGene Technologies, Inc.
The extensive list of expression cDNA libraries were the pivotal component of OriGenes DupLEX-A Yeast Two-Hybrid System kit. For each library, oligo(dT)-primerd cDNA was synthesized from high quality mRNAs and cloned unidirectionally between EcoRI and XhoI site of pJG4-5, the activation domain expression vector used in the DupLEX-A system...

See more about the dual hybrid system at: www.origene.com/reagents_tools/cdna_libraries/

The extensive list of expression cDNA libraries were the pivotal component of OriGenes DupLEX-A Yeast Two-Hybrid System kit. For each library, oligo(dT)-primerd cDNA was synthesized from high quality mRNAs and cloned unidirectionally between EcoRI and XhoI site of pJG4-5, the activation domain expression vector used in the DupLEX-A system. After plating the primary bacterial transformants, the cells were scraped from the plates, pooled, an aliquot was removed and frozen away and the plasmid was purified from the rest. Thus the researchers need not perform the laborious task of library DNA isolation. Moreover, the provided library DNA has never been amplified.

Although OriGene no longer provides the DupLEX-A System kit, we still make the libraries available to support the customers who have started using this methodology.

In addition, while designed to complement the yeast two-hybrid system, these libraries can be used as standard plasmid libraries for isolation of cDNA clones using conventional nucleic acid-based screening strategies.

See more about the dual hybrid system at: www.origene.com/reagents_tools/cdna_libraries/