Carryover Analysis Of GORE Protein Capture Devices With Two Monoclonal Antibodies

By Brad Joines, Mike McManaway and William Barrett, Ph.D., W. L. Gore & Associates, Inc.

Follow this ELISA testing performed according to kit instructions. The test samples were diluted with PBS to fall within the standard curve provided in the ELISA kits. To determine the presence of either monoclonal antibody within the test samples, the Adalimumab and Trastuzumab sets of samples were tested on both kits. Explore evidence based through ELISA assays that the GORE® Protein Capture Device did not have carryover after consecutive cycling of two different cell culture harvests when separated by a 15-minute sanitization cycle using 0.1 M NaOH.