BIOXYTECH LPO-586 Colorimetric Assay For Lipid Peroxidation Markers

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable, and decompose to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides generate malonaldehyde (MDA) and 4-hydroxyalkenals upon decomposition. Measurement of malonaldehyde and 4-hydroxyalkenals has been used as an indicator of lipid peroxidation (ESTERBAUER, 1991). The LPO-586 method is designed to assay either MDA alone (in hydrochloric acid ) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid).

Principles of the Procedure:

The LPO-586 assay is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole (R1) , with MDA and 4-hydroxyalkenals at 45°C. One molecule of either MDA or 4-hydroxyalkenal reacts with 2 molecules of reagent R1 to yield a stable chromophore with maximal absorbance at 586 nm.
REAGENTS
Warnings and Precautions

• Do not smoke, eat or drink in areas where samples and reagents are handled.
  
• Wear disposable gloves when handling samples and reagents.
  
• Do not pipette reagents or samples by mouth.
  
• In case of accidental exposure of skin, mucous membranes or eyes to the R1 or R2 reagents, thoroughly wash the exposed area with water.

FOR Research Use Only. Not for use in diagnostic procedures.
FOR in vitro Use Only

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