Biological And Process-Engineering Characterization Of The Minifors 2 Laboratory Bioreactor

This application note first describes a systematic characterization of a stirring reactor, based on the example of the Minifors 2. Using this as a starting point, we present examples of microbial cultivation of a methylotrophic yeast that demonstrate system performance at its technical limits.

In these studies data was collected showing the Minifors 2 is well suited for microbial applications. At the same time, scale-up should not pose any difficulty as the results from the three reactors were found to be highly comparable. On the whole, the system proved to be very well suited for physiological characterization of bioprocesses involving Pichia pastoris.