Best Practices For Scaling PSC Expansion From Bench To Pilot To Commercial Scale Using Vertical Wheel Bioreactors

Scaling pluripotent stem cell (PSC) expansion from the research bench to commercial manufacturing presents significant challenges, from maintaining cell quality to ensuring process reproducibility. The unique hydrodynamic environment of Vertical-Wheel Bioreactors provides a solution, creating a homogenous, low-shear culture favorable for sensitive cell types like PSCs. This system’s geometry and fluid dynamics are consistent across scales, enabling predictable, linear scale-up from 0.1L to 80L.

This webinar details the best practices established through hundreds of experiments, covering process characterization, developing a robust 2D seed train, and scalable medium exchange strategies like perfusion to avoid aggregate clumping. You’ll see the data from a world-first run that produced over 200 billion PSCs in a single 50L batch, demonstrating true linear scalability.

Watch the recording to learn how to implement these innovative strategies in your lab.