Best Practices For Multi-Use Bioreactor Sterilization

Bioreactors are commonly sterilized in simple laboratory sterilizers. In general, steam sterilizers operate in two modes, gravity or vacuum. Testing results demonstrate inefficient ambient air removal and subsequent inefficacy of sterilization in these two common methods. In a Sterilization Efficacy Evaluation completed by the Getinge Sterilization Test Lab, various bioreactor sterilization methods were studied.

This brief describes how a multi-use bioreactor, headplate, tubing, vent filters, liquid media and filled liquid addition bottle can be sterilized at once and in a single integrated process with confirmed sterilization efficacy.