Behind The Data: Next‑Generation Enzymes For Targeted Gene Insertion

Targeted insertion of large genetic cargo remains a key challenge for advanced cell therapies, particularly when complex edits or allogeneic applications are required. Large serine recombinases offer a promising path by integrating sizable payloads without exposed double-strand breaks. Screening more than 30,000 candidates revealed a diverse set of active enzymes, including many that exceeded established literature benchmarks. In therapeutically relevant cell types, selected recombinases achieved robust integration with favorable insertion profiles. When paired with CRISPR-enabled landing pads, the approach produced greater than 90% landing pad installation at the TRAC locus and more than 67% insertion of a CD19 CAR across multiple donors.

Learn how continued enzyme discovery, protein engineering, and AI-guided optimization could expand the possibilities for precise, multiplexed genome engineering.