Beckman Coulter Capillary Electrophoresis Demonstrated At Pittcon 2012
Beckman Coulter's Mark Lies talks about their latest capillary electrophoresis platform to be used in biopharmaceuticals for purity analysis and carbohydrate analysis. The concept is to apply analytical separations in a capillary on an automated system. Beckman Coulter offers validated chemistry where they're able to give you a box of chemistry with the tools to be able to separate based on size of a protein, charge of a protein, or look at the carbohydrate population associated with that protein.
Hi my name is Mark Lies with Beckman Coulter Life Sciences and I am the marketing manager for capillary electrophoresis products.
This is our PA 800 Plus Pharmaceutical Analysis System which is our latest CE platform that is really being used in a bio-pharmaceutical setting specifically for purity analysis, as well as glycan or carbohydrate analysis. The concept is to apply analytical separations in a capillary like this one on an automated system which you can see here where we actually have a cartridge into which that capillary is built allowing for on instrument, automated and quantitative separation based on a variety of different configured parameters.
The other thing that we offer with this platform is validated chemistry where we are able to give you a box of chemistry in which you have the tools to separate based on size of a protein for example, charge of a protein, or look at the carbohydrate population associated with that protein. Some of the benefits that you get with the PA 800 Plus system is the fact that it is a fully validated platform. It has become the gold standard in the biopharmaceutical industry for purity separations.
Some of the key features that you are seeing are on-board pressure handling system, on-board sample temperature control system, capillary cooling allowing you to get a greater reproducibility because the stability in the actual separation temperatures is being held, automation where you can run up to 96 well plates in terms of sample analysis.
Some of the detection systems — one you can see here — is our laser induced fluorescent detector allowing you to look at samples that have been labeled potentially with chlorophors using LIF excitation and detection. You can also you UV or diode array detection to look at standard UV absorbance data.
The other area that has really made this a simplified system in terms of biopharmaceutical analysis is in the software application. What we have done with the software is develop a system that makes it very simple, while still incorporating all of the key features of our 32 karat software platform.
What I am showing you here is essentially a simulation of a sample monoclonal antibody in this case that is being run. You would simply start by selecting an application — one of the applications that we have preloaded on the software and developing a configuration of samples and buffer vials, based on the number of controls or the number of standards that you might want to incorporate into your experiment, and then acquiring that data which you see can see is happening here all while showing you what is going on, what the sequence of that particular operation is and then the live data as it comes off of the instrument.