Automation Of The CRISPR- Based Cell Line Development Workflow

Typical cell-line development requires the screening of tens of thousands of clones to find the best stable cells that yield high amounts of bioproducts. Review an in-depth study of the automation of a mammalian cell-line development workflow—the automated, integrated screening of transfected cells for edits, monoclonality, and growth assessment. The integrated system used includes a single-cell printer, Clone Select Imager – Florescence (CSI-FL), a liquid handler, an automated incubator, hotels, barcode readers, and a collaborative robot.

Benefits of the system include:

• Quick scale-up acceleration
• Increase in walk-away time
• Consistent processing
• Reduce redundant tasks
• Better cellular monitoring
• Dynamic workflow solutions