Automation Of iPSC Culture, Passaging, And Expansion With The CellXpress.ai Automated Cell Culture System

By Oksana Sirenko, Emilie Keidel, Astrid Michlmayr, Marco Lindner, Angeline Lim, and Felix Spira

This content is brought to you by Molecular Devices, a Danaher Operating Company.

Induced pluripotent stem cell (iPSC)-derived models have become an invaluable tool in modern scientific research that enables researchers to generate a variety of cell types, organoids, and tissues. These stem cells are extensively utilized to create disease-specific phenotypes through CRISPR gene editing to facilitate the discovery of novel drug targets using patient-derived samples. Despite their immense potential, the process of culturing iPSCs is often hindered by labor-intensive and highly demanding steps.

To address these challenges, we have developed the CellXpress.ai™ Automated Cell Culture System. This innovative solution revolutionizes the cell culture process by automating every aspect with an integrated incubator, liquid handler, and AI-powered, image-based decision-making system. The hands-off approach of CellXpress.ai™ efficiently manages the demanding feeding and passaging schedules by continuously monitoring the development of cell cultures through periodic imaging and analysis. Leveraging advanced machine learning algorithms, the system can autonomously initiate passaging, perform endpoint assays, or undertake troubleshooting steps as needed.

Access the full application note to learn more.