Automation-Assisted Washing Of Multicellular 3D Spheroids

Efficient and reproducible washing of 3D multicellular spheroids is critical for high-quality imaging and endpoint analysis in cell biology workflows. Explore how automated aspiration and dispensing using the HydroSpeed™ plate washer streamlines the staining and fixation of HeLa-GFP spheroids in both 96- and 384-well microplates. Manual washing steps, often prone to variability and cell loss, are replaced with precise, programmable routines that maintain spheroid integrity and minimize residual volumes. The integration of Spark® Cyto imaging and SparkControl™ software enables real-time monitoring and quality control throughout the workflow. Results show improved consistency, reduced handling errors, and minimal well-to-well variability, even in high-throughput formats.

For labs working with 3D cultures, discover how this approach offers a scalable solution to enhance data reliability and operational efficiency.