Automated Sampling Of A Four Vessel Parallel CHO Cell Culture Using Bioprocess Autosampler

This application note evaluates an automated sampling approach for parallel bioreactor systems, specifically applied to CHO cell cultures and microbial fermentation processes. The system enables continuous, unattended sample collection across multiple bioreactors, addressing key challenges in upstream bioprocessing.

Key findings demonstrate how automated sampling:

• Maintains sterility and sample integrity through temperature-controlled storage
• Reduces manual intervention while ensuring consistent sampling intervals
• Supports extended culture monitoring, including during off-hours
• Preserves critical parameters like cell viability, metabolite levels, and monoclonal antibody production

The study includes comparative data on cell density and IgG titers, along with analysis of sample stability during storage. Implementation considerations for integration with existing bioreactor systems are also discussed. For complete experimental details and results, download the full application note.