Integration Of An ATF Device With A DASbox® Mini Bioreactor System For Cell Culture Perfusion At Small Scale

By Hubert Schwarz, Ye Zhang, and Veronique Chotteau

Upstream bioprocessing in perfusion mode, holds great promise for the industrial production of cells and biologics. In perfusion fresh medium is added constantly to the bioreactor, and the used medium is harvested while cells are retained in the bioreactor. This configuration allows nutrients to be replenished and byproducts to be removed.

A DASbox Mini Bioreactor System and glass bioreactors with a maximum working volume of 250 mL can be used to setup a perfusion bioprocess system at a small scale. That system is suitable for the cultivation of CHO and HEK293 cells in perfusion for a prolonged period. Explore this perfusion bioreactor setup, what parameters you should prioritize for your perfusion process, which cell lines and culture media are used, and different aeration strategies to optimize oxygen transfer to the bioreactor.