Analysis Of NIST Antibody On The Agilent ProteoAnalyzer System

Understanding the size heterogeneity and purity of monoclonal antibodies is essential for reliable biopharmaceutical development, and the NISTmAb standard plays a key role in benchmarking analytical methods. Explore how a modern, parallel-capillary electrophoresis system measures up against traditional single‑capillary CE‑SDS when evaluating critical quality attributes such as monomeric purity, glycan occupancy, and thioether levels. Side‑by‑side comparisons show close agreement with published NIST reference values, reinforced by clear resolution of key fragments under both reduced and non-reduced conditions. The data demonstrates how automated, high‑throughput separations can maintain analytical confidence while accelerating workflows.

Access the full application note to learn how you can strengthen and modernize your protein characterization strategies.