Analysis Of Free Drug Content In Antibody-Drug Conjugate Using 2D-LC/Q-TOF

By Yulan Bian

Antibody-drug conjugates (ADCs) demand precise characterization to ensure both safety and therapeutic performance, with free drug content standing out as a critical quality attribute. Even small amounts of unconjugated payload—arising from incomplete conjugation or degradation—can impact efficacy and increase toxicity risk. A multidimensional approach using two-dimensional LC coupled with quadrupole time-of-flight mass spectrometry enables a clearer view of these impurities. By pairing size exclusion chromatography with reversed-phase separation, this workflow delivers sensitive, streamlined detection without requiring manual protein precipitation. The result is a more efficient path to understanding free drug levels and related species, helping strengthen analytical confidence and support robust ADC development.

Explore how this approach can simplify workflows while improving data quality.