Anaerobic Cultivation Processes Of Probiotic Bacteria In The BioLector XT Microbioreactor

By Anna Korona, m2p-labs GmbH

This content is brought to you by Beckman Coulter, a Danaher Operating Company.

Anaerobic cultivation of probiotics is critical for replicating microbiome-like conditions and advancing research in gut health. This resource explores how high-throughput systems enable precise control of oxygen-sensitive organisms such as Lactobacillus and Bifidobacterium bifidum, which dominate the human intestinal microbiota and play a key role in pH regulation and pathogen suppression.

The approach combines microfluidic technology with direct nitrogen gassing to maintain strict anaerobic conditions while simultaneously managing pH and feeding strategies. Real-time monitoring of biomass, dissolved oxygen, and pH ensures accurate process control, while flexible fed-batch configurations allow optimization of growth and acid production. Technical validation using external oxygen sensors and biological validation with strict anaerobes confirm the reliability of this method for small-scale, high-throughput experiments.

For researchers focused on probiotics, microbiome studies, or anaerobic fermentation, this methodology offers a scalable, reproducible solution for cultivating oxygen-sensitive strains under controlled conditions.

Access the full application note to learn how integrated anaerobic control can accelerate your probiotic research.