An Overview Of Fluorescent Dyes

By Dr. Christoph Greb,Leica Microsystems

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A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP – genetically linked to the protein of interest. With the help of fluorescent dyes, fluorescence microscopy is not only restricted to proteins but can also be used to detect nucleic acids, glycans, and other structures.

Continue to explore immunofluorescence, FITC and TRITC, cyanines, DNA staining, and more.