An Alternative Two-Step Mixed-Mode Approach To A Monoclonal Antibody (mAb) Purification Process

By Sharon Bola, Jamie Greenwood II, William Rushton, Mackenzie Scherr

Traditional mAb purification often involves a multi-step process, including Protein A capture, intermediate, and polishing steps. This can be time-consuming and costly. By combining different interaction mechanisms into a single step, mixed-mode chromatography can significantly reduce the number of purification steps.

This study compared two two-step mAb purification processes using mixed-mode resins to a standard three-step process. The first used Nuvia aPrime 4A, a hydrophobic anion exchange resin, while the second employed CHT™ Ceramic Hydroxyapatite XT Media, a calcium affinity cation exchange resin.

Results showed that both mixed-mode approaches achieved high-purity mAbs with fewer processing units, demonstrating the efficiency and potential cost savings of this streamlined purification strategy.