Aerobic Cultivation Of High-Oxygen-Demanding Microorganisms In The BioLector XT Microbioreactor

By Noud Drummen, Application Scientist Beckman Coulter Life Sciences

This content is brought to you by Beckman Coulter, a Danaher Operating Company.

High oxygen transfer rates are critical for cultivating fast-growing microorganisms and achieving high cell densities in bioprocess development. This resource explores strategies for aerobic cultivation under oxygen-enriched conditions, focusing on organisms such as Escherichia coli and Vibrio natriegens.

These species exhibit rapid growth and high metabolic activity, making them ideal for industrial biotechnology but challenging to maintain without oxygen limitation. The content highlights how precise control of gassing composition, feeding profiles, and pH regulation can prevent metabolic shifts that lead to unwanted by-products like acetate. It also examines advanced approaches such as signal-triggered feeding based on dissolved oxygen levels, enabling near-continuous substrate supply without compromising aerobic metabolism. By leveraging microfluidic technology and adjustable oxygen concentrations, researchers can optimize fed-batch strategies, improve culture performance, and maintain process stability.

These insights provide a foundation for scaling high-density cultivations and enhancing productivity in applications ranging from recombinant protein production to metabolic engineering. Access the full resource to learn how oxygen-enriched cultivation strategies can transform microbial process optimization.