Many laboratories today are faced with producing timely and accurate results at reduced cost. The pace of life science R&D and manufacturing is being accelerated but the hours in a single day has not changed. The need for companies to shorten their development or manufacturing time means that process development must be fast and inexpensive without compromising the quality of the end product. To address this challenge, many companies are moving towards automation. This trend is definitely being seen in the chromatography space with the advent of multimodal chromatography process improvement and new multidimensional (multi-D) chromatography instruments. Although this concept has been in practice for decades, the instrumentation and software technology for multi-D chromatography continues to evolve — here we discuss what all the excitement is about.