Advancing Downstream Purification

Bispecifics, ADCs, and fusion proteins are pushing downstream teams past the standard mAb template, with tougher impurity profiles, variant separations, and aggregate control on top of pressure for speed and process economics. This application note looks at two directions for cation exchange: frontal-mode operation, where aggregates and host cell protein bind while the product flows through, and mixed-mode selectivity for hydrophobic, structurally complex formats. It pairs side-by-side data on resin and membrane formats with a fusion protein study that tested a manufacturer's existing three-step template against a compressed alternative.

See how the two frontal technologies differ and where feed aggregate content may point toward one over the other.