Advance Your Western Workflow Into The 21st Century With Abby

Protein analysis comes with many challenges, typically requiring labor-intensive, time-consuming protocols like the Western blot, which has multiple hands-on steps that increase user error and data variability. As a result, Western blot data is only semi-quantitative and does not provide highly reproducible immunoassay quantitation. Thus, there is a pressing need for an automated, reproducible, and quantifiable protein analysis method to replace the Western blot.

Abby™ is the latest Simple Western™ instrument from ProteinSimple, a Bio-Techne brand dedicated to changing the way scientists analyze proteins by eliminating common protein analysis workflow challenges. Abby automates Western analysis and delivers picogram-level sensitivity with her chemiluminescence detection. Abby separates your proteins by size and precisely manages antibody additions, incubations, washes, and even the detection steps. In just 3 hours, Abby generates fully analyzed, quantitated, and reproducible results with the ability to normalize target signals with Total Protein Detection.

In this Application Note, we show how Abby delivers picogram-level sensitivity, while the RePlex assay enables detection of multiple targets per sample as well as Total Protein Detection.