Adipose-derived Human Mesenchymal Cell Production In Corning® HYPERStack® 36-layer Cell Culture Vessels

By Hilary Sherman and Ann Ferrie, Corning Incorporated, Life Sciences

Mesenchymal stem cells (MSCs) can be isolated from a variety of tissue sources such as bone marrow, perinatal tissue, subcutaneous adipose tissue, and peripheral blood. However, adipose-derived tissue offers several advantages compared to other source materials; harvesting adipose tissue is less invasive compared to harvesting bone marrow, adipose tissue is more plentiful in the body, and its’ stem cell yield is higher5 as compared to bone marrow.

Here, we demonstrate the utility of the Corning HYPERStack 36-layer cell culture vessel as a tool to scale up and meet the growing demand of adipose-derived MSCs. Our results show that when seeded at 3,000 cells/cm² for four days, a single HYPERStack 36-layer vessel yields over 540 million human adipose-derived MSCs that exhibit high viability and express appropriate markers for MSC identity.