Addressing Host Cell Protein Challenges In Monoclonal Antibody Production

By Yair Peres, PhD, Field Application Scientist at Purolite

Purolite has developed a novel protein A-based agarose resin, Praesto Jetted A50 HipH, to address the challenges of host cell proteins (HCP) in monoclonal antibody production. HCP can affect the quality of the final product and potentially cause adverse immune responses in patients. This new resin allows for the purification of Fc-containing molecules under milder conditions, reducing protein aggregation and HCP levels. Studies have shown that this resin can increase HCP clearance by 2.3 to 8.6 times compared to traditional protein A resins, leading to unprecedented levels of HCP removal and enhanced product purity. Various research papers have explored the development of improved column wash steps for HCP clearance, the kinetics of protein aggregation, and potential downstream platform approaches for antibody production and purification.