Achieve Fast And Efficient Isolation Of Exosomes From Stem Cells

By Pascal Rowart, Vincent Dufey, Jan Knop, Françoise De Longueville

Extensive research is being done to understand and use exosomes due to their correlation with immune responses, central nervous system-related diseases, tumor genesis and cancer, the spread of viruses , and neurological disorders. In this study, exosome isolation was carried out by the High-Speed Centrifuge CR22N and the Ultracentrifuge CP100NX after culturing human adiposederived stem cells (hADSC) in suspension on microcarriers in the DASbox Mini Bioreactor System equipped with BioBLU 0.3c Single-Use Bioreactors. Review the demonstration of how a workflow consisting of this bioreactor and bioreactor systems combination, as well as high-speed and ultracentrifugation, is a viable combination for the successful collection of intact and homogeneous hADSC-derived exosomes.