Accurate Mass Calibration With MassFerence P2

Choosing the right mass photometry calibrant to streamline AAV characterization can allow you to optimize how you calculate the mass of the biomolecules in your sample. The MassFerence P2 is a mass photometry calibrant, recommended for use with the SamuxMP and SamuxMP Auto for AAV sample characterization. In this study, we see the results of tests to judge the repeatability of calibrations performed with MassFerence P2. A mixed sample was used in this testing that contained empty AAV8 capsids (3.7 MDa) and cross-linked thyroglobulin (TG) monomers (0.67 MDa) and dimers (1.34 MDa). To test the reliability of one-point calibration, mass photometry measurements calibrated with one-point (MassFerence P2), two-point (mix of MassFerence P2 and TG) or three-point (cross-linked TG) calibrations were all compared. Explore the results of this study into MassFerence P2's calibration performance.