AAV Purification Using AEX And Mixed-Mode Chromatography

Recombinant adeno‐associated viruses (rAAV) are among the most promising vectors for long‐term gene transduction. These viruses have a high degree of safety, making them ideal for gene therapy applications. Significant progress has been made in improving rAAV vector production and purification. In this webcast, we present a study exploring different chromatography workflow solutions to purify rAAV8. Experimental conditions and data will be discussed using anion exchange (AEX) and mixed-mode chromatography to purify rAAV8.

Key takeaways:

• Increased efficiencies using AEX for capture
• Mixed-mode chromatography used to separate empty capsid from full capsid and remove DNA
• More flexible, economical purification workflow compared to traditional purification process