A Tool For Process Development And Stem-Cell Derived Exosome Production In Standardized Culture Conditions

By Silvia Tejerina, Vincent Dufey, Jean-François Hoet, Aurélie Tacheny, and Françoise De Longueville, Eppendorf Application Technologies S.A

Exosomes, and more specifically stem cell-derived exosomes, are now of great interest as cell-free therapeutic tools due to their strong diagnostic and therapeutical potential in various diseases models including skin, nervous system, heart, liver, and kidney. However, to explore the use of exosomes in the different biomedical areas, large amounts of high-quality exosomes need to be produced. To achieve this challenge, the culture of cells in the controlled environment offered by stirred-tank bioreactors can facilitate the standardized expansion of high yield of viable cells and enhance the reproducible production of extracellular vesicles.

In this study, we use an DASbox Mini Bioreactor System equipped with BioBLU 0.3c Single-Use Bioreactors as a system to culture human ADSCs in suspension on microcarriers to produce exosomes. Read how this fully controlled system allows an expansion of high yield of viable cells and optimizes a production of high-quality extracellular vesicles in standardized culture conditions.