A Single-Use, Scale-Down, Predictive Solution For Intensified Perfusion Development

Perfusion is becoming a typical approach to achieving upstream process intensification. The ability to scale down these processes, however, has been limited due to a lack of appropriate technologies. Labs cannot run enough bench-scale 2L bioreactors with cell retention devices due to resource constraints and high throughput systems lacking the required perfusion or control capabilities.

Here, we describe a new microbioreactor system that fulfills this throughput-performance gap, providing single-use, perfusion bioreactors with 2mL working volume that can replicate bench-scale perfusion processes at industrially relevant cell densities.