A Scalable Immobilized Metal Affinity Chromatography Media For Process Purification

Downstream process purification of proteins requires a resin with optimized bead size for ideal pressure/flow properties and decent dynamic binding capacity DBC that provides production efficiencies and good process economics. This video explains how our newly developed metal chelate affinity resin — Nuvia IMAC — provides the mechanical strength, pore structures, ligand density, and particle size distribution required for an operation run at 300 cm/hr with a DBC of 40 mg/ml at 2 bar column backpressure. Protein purification can efficiently be scaled up from milligrams in the lab to kilograms in bioprocess manufacturing. The chemical stability of Nuvia IMAC Resins ensure efficient column regeneration, reproducibility between purification runs, extended column lifetime, or product purity. The resin is also compatible with reagents typically employed for histidine-tagged protein purification. In summary, Nuvia IMAC Resins overcome the challenges associated with process purification of proteins using metal affinity chromatography.