The first Protein A resins were based on the wild type of Protein A expressed by a pathogenic Staphylococcus aureus strain. Since then, the manufacturing of monoclonal antibodies has grown tremendously and they are now the most important group of molecules in the pharma industry. Along with this development, Protein A resins have been significantly improved both with respect to capacity, productivity, and alkaline stability, resulting in a dramatic enhancement of process performance.
Current trends in antibody-based therapeutics include the development of a variety of different atypical IgG constructs that in many cases are prone to aggregation, are expressed at very high titre, and contain several product variants. Therefore, there is a growing interest in resins capable of resolving product variants at the protein A step and eluting IgG at milder pH conditions when compared to other Protein A resins on the market.
Praesto ® Jetted A50 HipH has been designed specifically for the elution of Fc-containing molecules up to pH 5.
Developed in collaboration with Repligen Corporation, Praesto Jetted A50 HipH is the only protein A resin with this capability.
- Up to 60mg/ml dynamic binding capacity
- Elution up to pH5
- Resistant to 0.1 M NaOH for >100 CYCLES (15 min contact time)